J PLANT NUTR SOIL SC ：中科院成都生物所丨低氮提高云杉氮利用率新机制（附NMT实验体系）

转自中关村旭月非损伤微测技术产业联盟

期刊：Journal of Plant Nutrition And Soil Science
主题：低氮提高云杉氮利用率新机制
标题：Morphological and physiological responses of Picea asperata to different nitrogen availability and pH
影响因子：2.057
检测指标：NH₄⁺、NO₃^-、H⁺流速
检测样品：云杉幼苗根系
NH₄⁺、NO₃^-、H⁺流实验处理方法：50 or 1000 mM NH4NO3 with pH5 or pH7，发芽后生长8周用于测定
NH₄⁺、NO₃^-、H⁺流实验测试液成份：0.1mM KCl, 0.1mM CaCl2, pH5 or pH7
作者：中科院成都生物所尹春英、唐波

英文摘要

Soil nitrogen (N) availability and pH are two determinants affecting plant growth, both of which are influenced by long‐term N deposition. However, the physiological mechanism of plants response to the changes in soil N availability and pH are not fully understood.

To investigate the response of Picea asperata to both factors, seedlings of P. asperata were exposed to 50 or 1000 µM NH4NO3 with pH 5 or pH 7. In the current study, P. asperata, regardless of N availability and pH in growth medium, exhibited invariably a urn:x-wiley:14368730:media:jpln201900103-math-0001 preference. Lower root biomass, root : shoot mass ratio, total root length and area, and root vitality were detected in high N condition compared to those in low N supply, corresponding well to lower net influxes of urn:x-wiley:14368730:media:jpln201900103-math-0002 and urn:x-wiley:14368730:media:jpln201900103-math-0003 at the root surface in both pH treatments. These results indicate that P. asperata may employ an active‐forge strategy to exploit nutrient resources for growth under low N availability, probably by increased below‐ground carbon allocation and net influxes of urn:x-wiley:14368730:media:jpln201900103-math-0004 and urn:x-wiley:14368730:media:jpln201900103-math-0005.

Although low pH, to some extent may generate more malondialdehyde, P. asperata would enhance pH tolerance by increased detoxification, i.e., antioxidant enzymes (peroxidase), free proline and soluble protein as well as improved carbohydrate status (i.e., soluble sugar and starch).

中文摘要（谷歌机翻译）

土壤氮（N）的有效性和pH是影响植物生长的两个决定因素，这两个因素均受长期氮沉降的影响。但是，尚未完全了解植物对土壤氮素利用率和pH值变化的生理机制。

为了研究云杉对两种因素的响应，将云杉幼苗暴露于50或1000 µM NH4NO3，pH 5或pH 7。展示了urn：x-wiley：14368730：media：jpln201900103-math-0001偏好设置。与低氮供应相比，高氮条件下根系生物量，根：茎总质量比，总根长和面积以及根系活力较低，这与：x-wiley：14368730：培养基的净入渗量较低相对应。在两种pH处理中，根表面的jpln201900103-math-0002和urn：x-wiley：14368730：media：jpln201900103-math-0003这些结果表明，P。asperata可能采用主动锻造策略来开发养分资源，以在低氮可利用性下进行生长，这可能是由于地下碳分配增加以及骨净流入：x-wiley：14368730：media：jpln201900103-math -0004和urn：x-wiley：14368730：media：jpln201900103-math-0005。

尽管低pH值在一定程度上可能产生更多的丙二醛，但曲霉假单胞菌会通过增加解毒作用（即抗氧化酶（过氧化物酶），游离脯氨酸和可溶性蛋白以及改善的碳水化合物状态（即可溶性糖和淀粉））提高pH耐受性。

结果表明：无论pH是5或7，低N的NH4+吸收速率高于高N；不同pH的NH4+吸收速率并无显著差异（图b）。低N的NO3-吸收速率同样高于高N，pH 5时两种N的NO3-吸收速率均低于pH7（图c）。相同pH条件下，低N的H+外排速率高于高N；同样N水平下，不同pH的H+外排无显著差异（图d）。由此可见，云杉通过扩大吸收区域和提高吸收速率等形态和生理的一系列调控从而适应土壤中的限制性N环境。

文章链接：https://onlinelibrary.wiley.com/doi/10.1002/jpln.201900103

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Environ Exp Bot：河南农业大学丨水稻根部响应缺氧的分子机制研究（附NMT实验体系）

转自中关村旭月非损伤微测技术产业联盟

期刊：Environmental and Experimental Botany
主题：水稻根部响应缺氧的分子机制研究
标题：Comparative morphological and transcriptomic responses of lowland and upland rice to root-zone hypoxia
影响因子：3.712
检测指标：IAA、O2流速
检测样品：水稻（低地和高地两种）距根尖0,200, 300, 600, 900, 1200, 1500, 2500 μm
IAA、O₂流速流实验处理方法：10日龄幼苗分别在充足氧（4.5-6.0 mg·L−1）和缺氧（0.9-2.1 mg·L−1）条件下培养5d
IAA、O₂流速流实验测试液成份：0.1mM KCl, 0.1mM CaCl2, 0.1mM MgCl2, 0.3mM MES, pH 5.5
作者：河南农业大学赵全志、辛泽毓、刘娟

英文摘要

Lowland and upland rice, as for two ecotypes, that have been exhibited different tolerance levels under hypoxia conditions. However, the molecular mechanisms underlying rice root hypoxia tolerance between them are not fully understood.

This study was performed to assess the morphological, physiological and transcriptional changes of roots in one lowland rice YueFu (YF) and one upland rice IRAT109 (IR) genotype. A morpho-physiological analysis revealed that compared to IR, YF showed less reduction of root length, root and shoot biomass, formed more aerenchyma in the root, and kept more oxygen influxes in root under hypoxia conditions. Indole-3-acetic acid (IAA) fluxes patterns exhibited a different response to hypoxia in YF and IR.

The contents of IAA, ethylene and hydrogen peroxide were significantly increased in YF and IR, but nitric oxide significantly increased only in YF under hypoxic conditions. Subsequently, transcriptome analysis revealed that more differentially expressed genes (DEGs) were identified to respond to hypoxia in YF than IR. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that DEGs in both genotypes enriched in energy metabolism, aerenchyma formation, reactive oxygen species, and cell wall modification, whereas more related DEGs in YF significantly enriched in these pathways than IR. The specific DEGs in YF especially enriched in phytohormone metabolism and signaling, such as auxin, jasmonic acid, and ethylene, but the specific DEGs in IR especially enriched in photosynthesis. All these results demonstrate that YF is more tolerant to hypoxia than IR, and elucidate some specific mechanisms underlying the differential hypoxia tolerance in lowland and upland rice.

This study has provided valuable candidate genes for genetic improvement of rice in adapting to hypoxia stress.

中文摘要（谷歌机翻译）

就两种生态型而言，低陆和旱稻在低氧条件下表现出不同的耐受水平。然而，它们之间的水稻根缺氧耐受性的分子机制尚不完全清楚。

这项研究的目的是评估一种低陆稻悦富（YF）和一种陆稻IRAT109（IR）基因型的根的形态，生理和转录变化。形态生理学分析表明，与IR相比，YF在低氧条件下显示的根长，根和茎生物量减少减少，在根中形成更多的气孔，并保持更多的氧流入。吲哚-3-乙酸（IAA）通量模式在YF和IR中显示出对缺氧的不同响应。

在低氧条件下，IAF，乙烯和过氧化氢的含量在YF和IR中显着增加，而一氧化氮仅在YF中显着增加。随后，转录组分析显示，与IR相比，鉴定出更多的差异表达基因（DEG）对YF缺氧有反应。基因本体论（GO）和《京都基因与基因组百科全书》（KEGG）分析表明，两种基因型的DEG均富含能量代谢，通气组织形成，活性氧种类和细胞壁修饰，而YF中更多相关的DEG则显着丰富了这些途径比红外线YF中的特定DEGs尤其富含植物激素代谢和信号传导，例如生长素，茉莉酸和乙烯，而IR中的特定DEGs尤其富含光合作用。所有这些结果表明，YF比IR更耐缺氧，并阐明了低陆稻和旱稻差异耐缺氧性的某些特定机制。

该研究为水稻遗传适应低氧胁迫提供了有价值的候选基因。

结果表明：在氧气充足（Aer）的条件下，分生区吸收O₂比较剧烈。YF（低地品种）和IR（高地品种）在300μm处吸收的O2最多，其流速值分别达到79.14 pmol·cm⁻²·s⁻¹和83.72 pmol·cm⁻²·s⁻¹，而在缺氧（Hyp）条件下分别为44.77 pmol·cm⁻²·s⁻¹和40.08 pmol·cm⁻²·s⁻¹；IR明显降低的更多一些。这说明，分生区是水稻根部感受氧的关键区域，IR对缺氧条件更加敏感。
根分生区同时也是IAA较为敏感的区域。缺氧条件下，除IR的300μm处之外，两种水稻均表现为吸收IAA。氧气充足时，YF和IR在600μm处的IAA吸收速率分别为29,740 fmol·cm⁻²·s^-1和35,092 fmol·cm⁻²·s^-1；而在缺氧条件下分别为8864 fmol·cm⁻²·s^-1和5441 fmol·cm⁻²·s^-1。结合IAA含量测定结果，发现缺氧促使根部积累IAA。这说明生长素参与了缺氧条件下根系发育的调控过程。

文章链接：https://www.sciencedirect.com/science/article/pii/S0098847219315138?via%3Dihub

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Plant Cell Environ：北京林业大学丨沙冬青虫害/盐害交互抗性机制研究（附NMT实验体系）

转自中关村旭月非损伤微测技术产业联盟

期刊：Plant Cell & Environment
主题：沙冬青虫害/盐害交互抗性机制研究
标题：Herbivore exposure alters ion fluxes and improves salt tolerance in a desert shrub
影响因子：6.125
检测指标：Ca2+、K+、Na+、H+流速
检测样品：矮沙冬青
作者：北京林业大学沈应柏、陈迎迎

文章简介

沙冬青是西北荒漠唯一的常绿阔叶灌木，是第三纪孑遗植物，国家二级濒危物种。沙冬青生境极其恶劣，常伴随着极端温度、干旱、盐碱、虫害，但目前关于沙冬青生物与非生物交互抗性的研究甚少。因此，沈应柏教授研究团队利用非损伤微测、激光共聚焦等技术，系统研究了经昆虫取食的沙冬青在遭受盐胁迫时的根部离子跨膜转运模式。该研究发现，昆虫取食预处理可能通过激活OPR3活性诱导茉莉酸的积累，茉莉酸信号途径的激活诱导了胞内Ca2+的迅速积累，增强了质膜H+-ATPase活性，促进了胞内过多的Na+经由Na+/H+逆向转运体的外排，同时抑制K+的流失，有助于维持细胞内的K+/Na+平衡，最终增强沙冬青的耐盐性。

博士研究生陈迎迎为该论文第一作者，沈应柏教授为通讯作者。该研究相关工作得到国家自然科学基金项目（31270655）的资助。

英文摘要

Plants have evolved complex mechanisms that allow them to withstand multiple environmental stresses, including biotic and abiotic stresses.

Here, we investigated the interaction between herbivore exposure and salt stress of Ammopiptanthus nanus, a desert shrub. We found that jasmonic acid (JA) was involved in plant responses to both herbivore attack and salt stress, leading to an increased NaCl stress tolerance for herbivore-pretreated plants, and increase in K+/Na+ ratio in roots. Further evidence revealed the mechanism by which herbivore improved plant NaCl tolerance. Herbivore pretreatment reduced K+ efflux and increased Na+ efflux in plants subjected to long-term, short-term, or transient NaCl stress.

Moreover, herbivore pretreatment promoted H+ efflux by increasing plasma membrane H+-ATPase activity. This H+ efflux creates a transmembrane proton motive force that drives the Na+/H+ antiporter to expel excess Na+ into the external medium. In addition, high cytosolic Ca2+ was observed in the roots of herbivore-treated plants exposed to NaCl, and this effect may be regulated by H+-ATPase.

Taken together, herbivore exposure enhances A. nanus tolerance to salt stress by activating the JA signalling pathway, increasing plasma membrane H+-ATPase activity, promoting cytosolic Ca2+ accumulation, and then restricting K+ leakage and reducing Na+ accumulation in the cytosol.

中文摘要（谷歌机翻译）

植物已经进化出复杂的机制，可以承受多种环境胁迫，包括生物和非生物胁迫。

在这里，我们调查了草食动物暴露与沙漠灌木沙冬青盐胁迫之间的相互作用。我们发现茉莉酸（JA）参与了植物对食草动物侵袭和盐胁迫的反应，导致食草动物预处理过的植物对NaCl胁迫的耐受性增加，并且根中K+ / Na+比率增加。进一步的证据揭示了草食动物改善植物NaCl耐受性的机制。草食动物预处理可降低长期，短期或短暂NaCl胁迫下植物的K+流出量并增加Na +流出量。

此外，草食动物预处理通过增加质膜H+ -ATPase活性来促进H +流出。这种H+外流产生跨膜质子原动力，该原动力驱动Na+ / H+反向转运蛋白将过量的Na+排出到外部介质中。此外，在暴露于NaCl的食草动物处理过的植物的根部中观察到高的胞质Ca2+，这种作用可能受H+ -ATPase调节。

总之，通过激活JA信号传导途径，增加质膜H+ -ATPase活性，促进胞质Ca2 +积累，然后限制K+泄漏并减少细胞质中Na +的积累，草食动物暴露增强了南芥对盐胁迫的耐受性。

Figure 2. Effects of NaCl on the stable and transient flux of K+ along the A. nanus root axis (from 0 to 2000 μm from the root apex) with or without herbivore pretreatment. (A) The stable K+ flux was recorded along the axis of the root apex (0~2000 μm from the root tip) at 200-μm intervals, after long-term (LT) combined stresses (24-h HE + 7-d NaCl), salt stress (7-d NaCl), or no stress (control). (B) The bar chart represents the mean K+ flux value of all points along the roots following the four treatments. (C) The transient K+ flux kinetics were measured at the surface of the root, 600 μm from the tip, before and after the application of 100 mM NaCl. Three minutes of baseline data were recorded before NaCl application. The arrow indicates the time point of NaCl addition. Ten minutes of data were recorded after NaCl application. Samples were pretreated with tetraethylammonium (TEA), a K+ channel blocker, for 30 min before test. (D) The mean K+ flux was calculated before (pre-exposure), immediately after (peak-response), and 10 min after (post-exposure) the NaCl treatment. Different letters indicate significant differences at P ≤ 0.05 (Student’s t-test). Data were obtained from 5–7 A. nanus individuals. Error bars represent SE.

文章链接：https://onlinelibrary.wiley.com/doi/10.1111/pce.13662

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（一作视频讲解）Plant Cell Environ：浙江大学丨ABA可抑制超富集东南景天对Cd的吸收

转自中关村旭月非损伤微测技术产业联盟

一作视频讲解

期刊：Plant cell and environmen
主题：ABA可抑制超富集东南景天对Cd的吸收
标题：Abscisic acid‐mediated modifications of radial apoplastic transport pathway play a key role in cadmium uptake in hyperaccumulator Sedum alfredii
影响因子：5.415
检测指标：Cd²⁺流速
检测样品：东南景天根部距离根尖0mm至3mm，间隔0.25mm
Cd²⁺流速实验处理方法：9周龄的东南景天幼苗，分别用25 μM Cd(NO3)2、25 μM Cd(NO3)2+1 μM ABA、25 μM Cd(NO3)2+50 μM Abamine处理7天
Cd²⁺流实验测试液成份：推荐测试液：0.025mM CdCl2，0.1mM KCl，pH6.0
作者：浙江大学李廷强、陶琦

文章简介

ABA是植物抵抗重金属毒害的一种重要的植物激素。但是，ABA如何影响植物根的质体外运输途径以及重金属离子的吸收，这些过程机理还不明确。

2019年初，浙江大学李廷强教授团队在Plant, Cell & Environment上发表了脱落酸（ABA）抑制东南景天富集重金属镉（Cd）的研究成果，文章标题为“Abscisic acid‐mediated modifications of radial apoplastic transport pathway play a key role in cadmium uptake in hyperaccumulator Sedum alfredii”。李教授课题组通过比较两种东南景天在Cd处理下不同的生理变化及基因表达情况，发现在非超富集型（NHE）东南景天中，由于ABA合成基因（SaABA2，SaNCED）表达上调，根的内源性ABA含量升高，而超富集型（HE）东南景天中不存在这种变化。而且，NHE东南景天中内胚层凯氏带（CSs）和栓质层（SL）的沉积更靠近根尖。以上结果表明：ABA促进了苯丙氨酸解氨酶、过氧化物酶和栓质相关基因（SaCYP86A1, SaGPAT5, and SaKCS20）的表达，从而调控两种质体外屏障的沉积过程。

本研究利用基于非损伤微测技术的NMT活体生理检测仪Physiolyzer®，检测植物根部的Cd吸收速率，发现两种类型东南景天中，Cd的吸收随着距离根尖越远而逐渐减少；HE东南景天根尖区的Cd吸收总含量更高。不同条件处理下，Cd吸收的下降位点（图中黑色箭头表示）与根尖距离不同，且下降位点与CSs的起始位点一致。实验结果表明：ABA通过促进CSs和SL的沉积形成屏障，从而显著抑制Cd进入根内。

不同条件处理下，两种类型东南景天根部Cd2+吸收速率对比，负值表示吸收。

英文摘要

Abscisic acid (ABA) is a key phytohormone underlying plant resistance to toxic metals. However, regulatory effects of ABA on apoplastic transport in roots and consequences for uptake of metal ions are poorly understood.

Here, we demonstrate how ABA regulates development of apoplastic barriers in roots of two ecotypes of Sedum alfredii and assess effects on cadmium (Cd) uptake. Under Cd treatment, increased endogenous ABA level was detected in roots of nonhyperaccumulating ecotype (NHE) due to up‐regulated expressions of ABA biosynthesis genes (SaABA2, SaNCED), but no change was observed in hyperaccumulating ecotype (HE). Simultaneously, endodermal Casparian strips (CSs) and suberin lamellae (SL) were deposited closer to root tips of NHE compared with HE. Interestingly, the vessel‐to‐CSs overlap was identified as an ABA‐driven anatomical trait

Results of correlation analyses and exogenous applications of ABA/Abamine indicate that ABA regulates development of both types of apoplastic barriers through promoting activities of phenylalanine ammonialyase, peroxidase, and expressions of suberin‐related genes (SaCYP86A1, SaGPAT5, and SaKCS20). Using scanning ion‐selected electrode technique and PTS tracer confirmed that ABA‐promoted deposition of CSs and SL significantly reduced Cd entrance into root stele.

Therefore, maintenance of low ABA levels in HE minimized deposition of apoplastic barriers and allowed maximization of Cd uptake via apoplastic pathway.

中文摘要（谷歌机翻译）

脱落酸（ABA）是植物抵抗有毒金属的基础的重要植物激素。然而，人们对ABA对根部质外体运输的调节作用以及对金属离子吸收的后果了解甚少。

在这里，我们演示了ABA如何调节两种生态型苜蓿景天的根中的质外性障碍的发展，并评估对镉（Cd）吸收的影响。在Cd处理下，由于ABA生物合成基因（SaABA2，SaNCED）的表达上调，在非高累积生态型（NHE）的根中检测到内源ABA水平升高，但在高累积生态型（HE）中未观察到变化。同时，与HE相比，内膜Casparian条（CSs）和suberin lamellae（SL）沉积在NHE根尖附近。有趣的是，血管与CS的重叠被确定为ABA驱动的解剖特征。

相关分析和ABA /阿巴胺的外源应用的结果表明，ABA通过促进苯丙氨酸解氨酶，过氧化物酶的活性以及与地下蛋白相关基因（SaCYP86A1，SaGPAT5和SaKCS20）的表达来调节两种质外性障碍的发展。使用扫描离子选择电极技术和PTS示踪剂证实，ABA促进了CS和SL的沉积，显着减少了Cd进入根石碑。

因此，维持HE中低ABA水平可最大程度地减少质外性障碍的沉积，并允许通过质外途径最大程度地吸收Cd。

文章链接：https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.13506

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Plant J：港中大、山东农大丨水稻OsCBL10启动子天然变异影响种子萌发期的耐淹性（附NMT实验体系）

转自中关村旭月非损伤微测技术产业联盟

期刊：Plant journal
主题：水稻OsCBL10启动子天然变异影响种子萌发期的耐淹性
标题：Natural variation in the promoter of rice calcineurin B‐like protein10 (Os CBL 10) affects flooding tolerance during seed germination among rice subspecies
影响因子：5.775
检测指标：Ca²⁺流速
检测样品：水稻距胚芽鞘尖端不同距离（0、50、100、150、200、250、300、350、400、450、500和550μm）
Ca²⁺流速实验处理方法：水稻种子水淹胁迫
Ca²⁺流实验测试液成份：0.1 mM CaCl2、0.1 mM KCl、0.3 mM MES，pH 6.0
作者：香港中文大学张建华、山东农业大学刘鹰高、施璐

文章简介

2018年5月，香港中文大学张建华、山东农业大学刘鹰高教授课题组在Plant Journal杂志上发表题为“Natural variation in the promoter of rice Calcineurin B-like Protein10 (OsCBL10) affects flooding tolerance during seed germination among rice subspecies”的研究论文。

现代栽培水稻来源于旱稻的逐步演化，而这一演化过程的了解是揭示旱稻如何获得水淹抗性的关键，对于其它植物的耐水淹育种研究具有借鉴意义。

该研究通过分析水稻和旱稻的钙调磷酸酶B类蛋白（OsCBL10）的启动子区域发现两者存在差异。耐水淹的水稻品种都存在同样的T-Type类型启动子序列，此类型启动子会通过影响OsCBL10表达进而调节水淹胁迫下的Ca2+信号。

该项研究中，利用NMT技术检测了两个水稻栽培品种Low88和Up221胚芽鞘以及距离胚芽鞘尖端不同距离部位的Ca2+流速，结果显示两个品种直接的Ca²⁺流速存在差异。

结果表明：水淹胁迫下水稻胚芽鞘的Ca2+流速变化。

英文摘要

Rice (Oryza sativa L.) has two ecotypes, upland and lowland rice, that have been observed to show different tolerance levels under flooding stress.

In this study, two rice cultivars, upland (Up221, flooding‐intolerant) and lowland (Low88, flooding‐tolerant), were initially used to study their molecular mechanisms in response to flooding germination. We observed that variations in the OsCBL10 promoter sequences in these two cultivars might contribute to this divergence in flooding tolerance. Further analysis using another eight rice cultivars revealed that the OsCBL10 promoter could be classified as either a flooding‐tolerant type (T‐type) or a flooding‐intolerant type (I‐type).

The OsCBL10 T‐type promoter only existed in japonica lowland cultivars, whereas the OsCBL10 I‐type promoter existed in japonica upland, indica upland and indica lowland cultivars. Flooding‐tolerant rice cultivars containing the OsCBL10 T‐type promoter have shown lower Ca²⁺ flow and higher α‐amylase activities in comparison to those in flooding‐intolerant cultivars.

Furthermore, the OsCBL10 overexpression lines were sensitive to both flooding and hypoxic treatments during rice germination with enhanced Ca2+ flow in comparison to wild‐type. Subsequent findings also indicate that OsCBL10 may affect OsCIPK15 protein abundance and its downstream pathways.

In summary, our results suggest that the adaptation to flooding stress during rice germination is associated with two different OsCBL10 promoters, which in turn affect OsCBL10 expression in different cultivars and negatively affect OsCIPK15 protein accumulation and its downstream cascade.

中文摘要（谷歌机翻译）

水稻（Oryza sativa L.）有两种生态型，旱稻和低陆稻，在洪水胁迫下它们表现出不同的耐受水平。

在这项研究中，最初使用两个水稻品种，高地（Up221，耐水）和低地（Low88，耐水）来研究其对洪水萌发的分子机制。我们观察到，在这两个品种中，OsCBL10启动子序列的变异可能导致这种泛洪耐受性的差异。使用另外八个水稻品种的进一步分析表明，OsCBL10启动子可以分为耐水型（T型）或耐水型（I型）。

OsCBL10 T型启动子仅存在于粳稻低地品种中，而OsCBL10 I型启动子则存在于粳稻高地，in稻高地和low稻低地品种中。与耐水不耐旱品种相比，含有OsCBL10 T型启动子的耐水耐旱水稻品种显示出较低的Ca²⁺流量和较高的α-淀粉酶活性。

此外，与野生型相比，OsCBL10过表达品系对水稻发芽期间的水淹和低氧处理均敏感，且Ca2+流量增加。随后的发现还表明，OsCBL10可能会影响OsCIPK15蛋白的丰度及其下游途径。

总而言之，我们的结果表明，水稻发芽过程中对洪水胁迫的适应与两个不同的OsCBL10启动子有关，这反过来影响了OsCBL10在不同品种中的表达，并对OsCIPK15蛋白的积累及其下游级联反应产生了负面影响。

文章链接：https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.13881

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