重金属研究NMT标准化方案丨中关村认证实验标准

为什么会被非损伤微测技术所吸引用于植物重金属研究？

JXB、PCE非损伤微测技术重金属胁迫研究案例

NMT与重金属胁迫如何结合?工程师带您一探究竟

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1）C2019-023，熊杰，浙江理工大学，Rice，水稻，根（距离根尖200μm，500μm），O₂、Cd²⁺

Aeration Increases Cadmium (Cd) Retention by Enhancing Iron Plaque Formation and Regulating Pectin Synthesis in the Roots of Rice (Oryza sativa) Seedlings

2）C2019-024，柴团耀，中国科学院大学生命科学学院，BMC Plant Biol ，酵母菌、大肠杆菌，酵母细胞，大肠杆菌细胞，Cd²⁺

A repeat region from the Brassica juncea HMA4 gene BjHMA4R is specifically involved in Cd²⁺ binding in the cytosol under low heavy metal concentrations

3）C2019-032，张振华，湖南农业大学，plant sci，Cd²⁺、NO₃^-

Balance between nitrogen use efficiency and cadmium tolerance in Brassica napus and Arabidopsis thaliana

4）C2018-030，廉菲，江南大学，Sci Total Environ，水稻，根冠、伸长区、根毛区，Cd²⁺

The effect of biochar nanoparticles on rice plant growth and the uptake of heavy metals: Implications for agronomic benefits and potential risk

5）C2018-047，周功克，中科院青岛生物能源与过程研究所，Chemosphere，浮萍，根（根尖、距离根尖400μm，1000μm）
叶（顶点、节点、顶点与节点中间的位置），Cd²⁺

Comparative transcriptome analysis of duckweed (Landoltia punctata) in response to cadmium provides insights into molecular mechanisms underlying hyperaccumulation

6）C2018-052，李银心，中科院植物研究所，Plant Biotechnol J，甜高粱，根部（距离根尖300μm），Cd²⁺

Comparative transcriptome combined with morphophysiological analyses revealed key factors for differential cadmium accumulation in two contrasting sweet sorghum genotypes

7）C2018-059，李廷强，浙江大学，Plant Cell Environ，东南景天，根部距离根尖2.5mm至3mm，Cd²⁺

Abscisic acid‐mediated modifications of radial apoplastic transport pathway play a key role in cadmium uptake in hyperaccumulator Sedum alfredii

8）C2019-001，潘科，深圳大学，Chemosphere，硅藻，藻细胞，Cd²⁺

Salinity-dependent nanostructures and composition of cell surface and its relation to Cd toxicity in an estuarine diatom

9）C2019-013，张伟、高建伟，山东大学、山东省农科院，Plant Physiology，拟南芥，根（距离根尖400μm），Cd²⁺

Mitochondrial Pyruvate Carriers Prevent Cadmium Toxicity by Sustaining the TCA Cycle and Glutathione Synthesis

10）C2019-034，王果，福建农林大学，Chemosphere，Cd²⁺

Chlorine weaken the immobilization of Cd in soil-rice systems by biochar

11）C2017-034，李芳柏，广东省科学院生态环境研究所，Environmental Pollution，悬浮细胞，Cd²⁺

Silica nanoparticles alleviate cadmium toxicity in rice cells: Mechanisms and size effects

12）C2015-003，王荔军，华中农业大学，New Phytologist，悬浮细胞，Cd²⁺

A hemicellulose-bound form of silicon inhibits cadmium ion uptake in rice (Oryza sativa) cells

13）C2015-001，罗志斌，西北农林科技大学，New Phytologist，根，Ca²⁺/Cd²⁺/H⁺

Overexpression of bacterial gamma-glutamylcysteine synthetase mediates changes in cadmium influx, allocation and detoxification in poplar

14）C2013-019，王荔军，华中农业大学，New Phytologist，悬浮细胞，Cd²⁺

Inhibition of cadmium ion uptake in rice (Oryza sativa) cells by a wall-bound form of silicon

15）C2013-015，罗志斌，西北农林科技大学，PLANT CELL ENVIRON ，根，Cd²⁺H⁺

Ectomycorrhizas with Paxillus involutus enhance cadmium uptake and tolerance in Populus × canescens

16）C2012-017，徐进，中科院遗传与发育生物学研究所农业资源研究中心，New Phytologist，根，Cd²⁺

Comparative physiological responses of Solanum nigrum and Solanum torvum to cadmium stress

17）C2012-016，徐进，中科院遗传与发育生物学研究所农业资源研究中心，New Phytologist，根，Cd²⁺

Comparative transcriptome analysis of cadmium responses in Solanum nigrum and Solanum torvum

18）C2012-009，赵福庚，南京大学，PLANT CELL ENVIRON ，根，K⁺/Ca²⁺

Cadmium impairs ion homeostasis by altering K⁺ and Ca²⁺ channel activities in rice root hair cells

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Cd²⁺、Pb²⁺、Cu²⁺、Ca²⁺、H⁺

1）Pb²⁺、Cu²⁺生理功能概述

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2）科研案例

以三种常见湿地植物为材料，芦苇在距根尖大约0-200μm处为Cu²⁺释放区域，而在0-500μm处表现出Pb²⁺和Cd²⁺净外排。尽管在芦苇根部的较老部分中有Cu²⁺，Pb²⁺和Cd²⁺的内流，但总流入量比香蒲或印度香豆小得多。穿过根细胞质膜的这种减少的金属离子吸收和/或增加的金属离子外流，可能在一定程度上解释了芦苇对金属的更高抗性。

Li, L., Yu, S., Peijnenburg, W.J.G.M. et al. Determining the fluxes of ions (Pb²⁺, Cu²⁺ and Cd²⁺) at the root surface of wetland plants using the scanning ion-selective electrode technique. Plant Soil 414, 1–12 (2017).