OSense O-Sense



Plant Cell 南农:(H2O2流实验体系)硫化氢参与调节ABA诱导气孔关闭的分子机制


期刊:Plant Cell
标题:Persulfidation-based Modification of Cysteine Desulfhydrase and the NADPH Oxidase RBOHD Controls Guard Cell Abscisic Acid Signaling
H2O2流实验处理方法:4周龄的拟南芥幼苗,10μM ABA / 100μM NaHS瞬时处理
H2O2流实验测试液成份:10 mM MES,10 mM KCl,pH 6.15



Accumulating evidence suggests that hydrogen sulfide (H2S) is a gaseous signaling molecule that regulates diverse cellular signaling pathways through persulfidation, which involves the post translational modification (PTM) of specific cysteine residues to form persulfides. However, the mechanisms that underlie this important redox-based PTM remain poorly understood in higher plants.

We have, therefore, analyzed how protein persulfidation acts as a specific and reversible signaling mechanism during the plant abscisic acid (ABA) response. Here we show that ABA stimulates the persulfidation of L-cysteine desulfhydrase 1 (DES1), an important endogenous H2S enzyme, at Cys44 and Cys205 in a redox-dependent manner. Moreover, sustainable H2S accumulation drives persulfidation of the NADPH oxidase respiratory burst oxidase homolog protein D (RBOHD) at Cys825 and Cys890, which enhance its ability to produce reactive oxygen species. Physiologically, S-persulfidation-induced RBOHD activity is relevant to ABA-induced stomatal closure.

Together, these processes form a negative feedback loop that fine-tunes guard cell redox homeostasis and ABA signaling. These findings not only expand our current knowledge of H2S function in the context of guard cell ABA signaling, but also demonstrate the presence of a rapid signal integration mechanism involving specific and reversible redox-based PTMs that occur in response to changing environmental conditions.





(B) ABA- and NaHS-induced net H2O2 influxes in guard cells of rbohD and pCAB3:RBOHD rbohD plants. Leaves from 4-week-old plant were preincubated for 3 hr in opening buffer (10 mM MES, pH 6.15, and 10 mM KCl) under light (120 μE m−2s−1) and washed in MES buffer three times for 15 min each. The net H2O2 influxes were observed after ABA (10 μM) or NaHS (100 μM) treatment during the indicated times (n = 6)