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J Exp Bot :江苏师范大学丨多倍体维持钠钾稳态促耐盐能力的新机制(附NMT实验体系)

转自中关村旭月非损伤微测技术产业联盟

 

NMT是基因功能的活体检测技术,已被31位诺贝尔奖得主所在单位,及北大、清华、中科院使用。

2019年10月江苏师大NMT合作研究中心(测试中心)服务信息

江苏师范大学揭示多倍体植物盐胁迫下钾钠平衡调控机制

  • 期刊:Journal of Experimental botany
  • 主题:多倍体维持钠钾稳态促耐盐能力的新机制
  • 标题:Root-zone-specific sensitivity of K+-and Ca2+-permeable channels to H2O2 determines ion homeostasis in salinized diploid and hexaploid Ipomoea trifida
  • 影响因子:5.354
  • 检测指标:K+、H+、Ca2+、Na+流速
  • 检测部位:甘薯
    K+、H+、Ca2+瞬时:分生区(距离根尖500μm处),伸长(距尖端3毫米),成熟(尖端15毫米)
    K+、Na+:分生组织区(距离尖端300-600μm)测量间隔为100μm),伸长区(1-3 mm)测量间隔为500μm的尖端和成熟区(距尖端10-15毫米,测量间隔为1毫米)
  • K+、H+、Ca2+、Na+流速流实验处理方法::甘薯幼苗,150mM NaCl处理7d/150mM NaCl瞬时处理
  • K+、H+、Ca2+、Na+流速流实验测试液成份:
    H+/K+/Ca2+瞬时:0.1 mM NaCl, 0.1 mM MgCl2,0.1 mM CaCl2, and 0.5 mM KCl at pH 5.7
    K+:150 mM NaCl, 0.1 mM MgCl2, 0.1 mM CaCl2, and 0.5 mM KCl, pH 5.7
  • 作者:江苏师范大学孙健、李宗芸

英文摘要

Polyploids generally possess superior K+/Na+ homeostasis under saline conditions compared with their diploid progenitors.

In this study, we identified the physiological mechanisms involved in the ploidy-related mediation of K+/Na+ homeostasis in the roots of diploid (2x) and hexaploid (6x; autohexaploid) Ipomoea trifida, which is the closest relative of cultivated sweet potato. Results showed that 6x I. trifida retained more K+ and accumulated less Na+ in the root and leaf tissues under salt stress than 2x I. trifida.

Compared with its 2x ancestor, 6x I. trifida efficiently prevents K+ efflux from the meristem root zone under salt stress through its plasma membrane (PM) K+-permeable channels, which have low sensitivity to H2O2. Moreover, 6x I. trifida efficiently excludes Na+ from the elongation and mature root zones under salt stress because of the high sensitivity of PM Ca2+-permeable channels to H2O2.

Our results suggest the root-zone-specific sensitivity to H2O2 of PM K+- and Ca2+-permeable channels in the co-ordinated control of K+/Na+ homeostasis in salinized 2x and 6x I. trifida. This work provides new insights into the improved maintenance of K+/Na+ homeostasis of polyploids under salt stress.

中文摘要(谷歌机翻)

与二倍体祖细胞相比,多倍体在盐水条件下通常具有优越的K+ / Na+稳态。

在这项研究中,我们确定了与二倍体(2x)和六倍体(6x;自六倍体)Tripoda的根的K+/ Na+稳态的倍性相关介导有关的生理机制,这是栽培红薯的近亲。结果表明,与2x I. trifida相比,在盐胁迫下6x I. trifida保留更多的K +并在根和叶组织中积累较少的Na+。

与2x祖先相比,6x裂叶线虫通过其对H2O2敏感性低的质膜(PM)K+渗透通道有效地防止了盐胁迫下分生组织根区的K+流出。此外,由于PM Ca2+的可渗透通道对H2O2的敏感性高,因此6x I. trifida有效地将Na+从盐胁迫下的伸长和成熟根区中排除。

我们的结果表明,在盐渍化2x和6x盐粉虱的K+ / Na+动态平衡控制中,PM K+-和Ca2+渗透通道对H2O2的根区特异性敏感性。这项工作为改善盐胁迫下多倍体K+ / Na+稳态的维持提供了新的见解。

结果表明:在分生组织区中,观察到更高的K+流出量的是2x,在盐处理(约30分钟)期间,盐诱导的K+渗漏的平均速率高达4800 pmol cm-2 s-1(比6x高1.9倍)。但是,在伸长和成熟的根部区域观察到相反的趋势,其中6x比2x表现出更强烈的K+外排。

文章链接:https://academic.oup.com/jxb/article/70/4/1389/5301728