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SCI REP-UK :山东农大丨磁处理对盐胁迫下杨树氮吸收与分布的影响(附NMT实验体系)

转自中关村旭月非损伤微测技术产业联盟

 

 

  • 期刊:Scientific Reports
  • 主题:磁处理对盐胁迫下杨树氮吸收与分布的影响
  • 标题:The effects of magnetic treatment on nitrogen absorption and distribution in seedlings of Populus × euramericana ‘Neva’ under NaCl stress
  • 影响因子:4.011
  • 检测指标:NH4+、NO3-流速
  • 检测部位:杨树根部(伸长区距离根尖15mm)叶肉细胞
  • NH4+、NO3-流速流实验处理方法:杨树幼苗,在0g/L NaCl+磁化(M0),0g/L NaCl+非磁化(NM0),4g/L NaCl+磁化(M4),4g/L NaCl+非磁化(NM4)中处理
  • NH4+、NO3-流速流实验测试液成份:NH4+: 0.1 mM NH4NO3, 0.1 mM CaCl2, 0.3 mM MES, pH 5.5 NO3-: 0.1 mM NH4NO3, 1.0 mM KCl, 0.1 mM CaCl2, 0.3 mM MES, pH 5.5
  • 作者:山东农业大学林学院马风云、刘秀梅

英文摘要

A potted experiment with Populus × euramericana ‘Neva’ was carried out to assess whether there are positive effects of magnetic treatment of saline water (MTSW) on nitrogen metabolism under controlled conditions in a greenhouse.

Growth properties, nitrogen contents, enzyme activities and metabolite concentrations were determined based on field experiments and laboratory analysis after a 30-day treatment.

The results were as follows: (1) Biomass accumulation, root morphological properties and total nitrogen content were improved by MTSW. (2) Magnetization led to a greater increase in nitrate-nitrogen (NO3-N) content in roots than in leaves, accompanied by greater NO3 efflux and activated nitrate reductase. (3) MTSW led to a higher ammonium-nitrogen (NH4+-N) content and greater uptake of net NH4+ in the leaves than that in the roots. (4) Magnetization stimulated glutamine synthase, glutamate dehydrogenase and glutamate synthase activities, whereas the concentrations of glutathione and oxidized glutathione were increased in leaves but decreased in roots, and the total glutathione content was increased.

Overall, these results indicated some beneficial impacts of MTSW on nitrogen translocation under field conditions, especially for equilibrating the distribution of NO3-N and NH4+-N. Moreover, these findings confirmed the potential of using low-quality water for agriculture.

中文摘要(谷歌机翻)

进行了欧洲杨(Populus×euramericana'Neva')的盆栽试验,以评估在温室中受控条件下盐水(MTSW)磁处理对氮代谢的积极影响。

在30天处理后,基于田间实验和实验室分析确定生长特性,氮含量,酶活性和代谢物浓度。

结果如下:(1)MTSW提高了生物量积累,根系形态特征和总氮含量。(2)磁化导致根中硝态氮(NO3-N)含量比叶片更大,伴随着更多的NO3-外排和活化的硝酸还原酶。(3)MTSW导致叶片中铵态氮(NH4+-N)含量较高,净NH4+吸收量高于根系。 (4)磁化刺激谷氨酰胺合成酶,谷氨酸脱氢酶和谷氨酸合酶活性,而谷胱甘肽和氧化型谷胱甘肽的浓度在叶片中增加但在根中减少,总谷胱甘肽含量增加。

总体而言,这些结果表明MTSW对田间条件下氮转运的一些有益影响,特别是对于平衡NO3- N和NH4+-N的分布。此外,这些发现证实了使用劣质水进行农业的潜力。

结果表明:使用NMT技术监测叶肉细胞中和距离根尖15mm的伸长区NO3- 净流速,两者均显示外排。在测量的溶液中,叶肉细胞的NO3- 外排从235.09增加到290.23 pmol cm-2 s-1(图A),暴露于NaCl溶液中,细根中从123.53到157.51 pmol cm-2 s-1显着增加(p <0.05;图B)。在暴露于盐胁迫的植物中这些值比在对照中更高。当暴露于盐水溶液时,叶片和根部的NO3- 流速增加,并且叶肉细胞中的外排量大于根伸长区域中的外排量。与非磁处理组中的流速相比,NO3- 流速在暴露于磁场条件下的叶肉细胞中显示出显着更高的速率。吸收最大的为M4(290.23 pmol cm-2 s-1),最低吸收量为NM0(181.42 pmol cm-2 s-1)。然而,NO3- 流速在根中表现出相反的模式,在伸长区域中的外排显着降低;NO3- 最高外排为NM4(157.51 pmol cm-2 s-1),最低外排量为M0(38.05 pmol cm-2 s-1)

结果表明:NH4+的净流速不同于NO3-;叶肉细胞为吸收趋势(图A),根部为外排趋势(图B)。叶中NH4+的吸收显示出较低的绝对值。暴露于NaCl溶液(M4,NM4)的样品和暴露于对照处理(M0,NM0)的样品相比,M0显示出最大的流入量(-2518.85pmol cm-2 s-1),M4显示出第二大流出量(1164.15pmol cm-2 s-1),他们有着显着不同(p<0.05)。 此外,NM4中NH4+通量的绝对值与NM0非常接近,并且它们彼此没有差异。相反,盐胁迫下新生长的细根中NH4+的外排高于对照中观察到的,并且M4显示出最高的外排量(186.83 pmolcm-2 s-1),与M0相比显着不同(p <0.05)。如在叶中观察到的,NM4中的NH4+外排与根中的NM0相似,并且数值没有差异。与盐水处理的效果不同,磁处理诱导NH4+的外排比非磁处理更大,并且M4和M0都显示出比NM4和NM0更高的值。这两个因素之间也存在显着的相互作用(p <0.05)

文章链接:https://www.nature.com/articles/s41598-019-45719-6