NMT历史上的今天丨Plant Science 野大麦对盐胁迫时间的Na+、K+协同响应文章发表

转自中关村旭月非损伤微测技术产业联盟

NMT历史上的今天

2012年8月16日，中国农科院兰州畜牧与兽药研究所王晓力副研究员同兰州大学张金林副教授利用NMT在Plant Science上发表了标题为The coordinated regulation of Na⁺ and K⁺ in Hordeum brevisubulatum responding to time of salt stress的研究成果。

文献简介野大麦对盐胁迫时间的Na+、K+协同响应

期刊：Plant Science
主题：响应盐胁迫时间的大麦Na+和K+的调控
标题：The coordinated regulation of Na+and K+ in Hordeum brevisubulatum responding to time of salt stress
影响因子：3.362
检测指标：Na⁺，K⁺流速
检测部位：野大麦根尖部位
Na⁺，K⁺流实验处理方法：四叶期大麦，0/100mM NaCl处理7天，100mM NaCl处理60天
Na⁺，K⁺流实验测试液成份：0.1 mMKCl, 0.1 mM CaC12, 0.1 mM MgC12, 0.5 mM NaCl, 0.2 mM Na2SO4,0.3 mM MES, pH 6.0
通讯作者：中国农科院兰州畜牧与兽药研究所王晓力、兰州大学张金林

英文摘要

Hordeum brevisubulatum, called as wild barley, is a useful monocotyledonous halophyte for soil improvement in northern China. Although previously studied, its main salt tolerance mechanism remained controversial.

The current work showed that shoot Na⁺ concentration was increased rapidly with stress time and significantly higher than in wheat during 0–168 h of 100 mM NaCl treatment. Similar results were also found under 25 and 50 mM NaCl treatments. Even K⁺ was increased from 0.01 to 50 mM in the cultural solution, no significant effect was found on tissue Na⁺ concentrations. Interestingly, shoot growth was improved, and stronger root activity was maintained in H.

brevisubulatum compared with wheat after 7 days treatment of 100 mM NaCl. To investigate the long-term stress impact on tissue Na⁺, 100 mM NaCl was prolonged to 60 days. The maximum values of Na⁺ concentrations were observed at 7th in shoot and 14th day in roots, respectively, and then decreased gradually. Micro-electrode ion flux estimation was used and it was found that increasing Na⁺ efflux while maintaining K⁺ influx were the major strategies to reduce the Na+ concentration during long-term salt stress. Moreover, leaf Na⁺ secretions showed little contribution to the tissue Na⁺ decrease.

Thereby, the physiological mechanism for H. brevisubulatum to survive from long-term salt stress was proposed that rapid Na+ accumulation occurred in the shoot to respond the initial salt shock, then Na⁺ efflux was triggered and K⁺ influx was activated to maintain a stable K⁺/Na⁺ ratio in tissues.

中文摘要（谷歌机翻）

大麦（Hordeum brevisubulatum），被称为野生大麦，是一种有用的单子叶植物盐生植物，用于中国北方的土壤改良。虽然以前研究过，但其主要的耐盐机制仍存在争议。

目前的工作表明，在100mM NaCl处理0-168小时期间，芽Na⁺浓度随胁迫时间迅速增加，并显着高于小麦。在25和50mM NaCl处理下也发现了类似的结果。甚至K⁺在培养液中从0.01增加到50mM，对组织Na⁺浓度没有显着影响。有趣的是，枝条生长得到改善，并且在H.中保持了更强的根系活力。

在处理100mM NaCl 7天后，将brevisubulatum与小麦进行比较。为了研究长期应激对组织Na⁺的影响，将100mM NaCl延长至60天。在根中第7天和第14天分别观察到Na⁺浓度的最大值，然后逐渐下降。使用微电极离子通量估计并且发现在保持K⁺流入的同时增加Na⁺流出是在长期盐胁迫期间降低Na+浓度的主要策略。此外，叶Na+分泌物对组织Na+的贡献很小。

因此，提出了H. brevisubulatum在长期盐胁迫下存活的生理机制，即在芽中发生快速Na⁺积累以响应初始盐胁迫，然后触发Na⁺流出并激活K+流入以维持稳定的K⁺/Na⁺组织中的比例。

Fig. 4. Net Na+ ﬂux of H. brevisubulatum test by MIFE between 50 and 390 s in mea-suring solution after 0 and 100 mM NaCl treatment (increased stepwise with 25 mM per 12 h) in modiﬁed Hoagland solution for 7 d and 100 mM NaCl for 60 d. Roots (with shoots retained) were incubated in the measuring solution to equilibrate for 15 min in advance. Steady-state ion ﬂuxes were then recorded until the values variation amplitude is relatively stable. Ten plants for H. brevisubulatum were pooled in each replicate (n = 6). Values are means ± SD and bars indicate SD.

文章链接：https://www.sciencedirect.com/science/article/pii/S0168945216303193?via%3Dihub

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