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NMT历史上的今天丨New Phytol 纳米颗粒提升植物镉耐受性成果发表



2012年8月13日,华中农业大学王荔军、刘建利用NMT在Physiologia Plantarum上发表了标题为Inhibition of cadmium ion uptake in rice (Oryza sativa) cells by a wall-bound form of silicon的文章。


  • 期刊:New Phytologist
  • 主题:通过壁结合形式的硅抑制水稻(Oryza sativa)细胞中镉离子的吸收
  • 标题:Inhibition of cadmium ion uptake in rice (Oryza sativa) cells by a wall-bound form of silicon
  • 影响因子:6.736
  • 检测指标:、Cd2+流速
  • 检测部位:水稻根(距离根尖0、200、400、600、800、2000μm)、悬浮细胞
  • Cd2+流实验处理方法:一个月的水稻幼苗,0、1mM 硅酸处理2个月(由固体培养基转至液体培养基后)
  • Cd2+流实验测试液成份:0.03mM CdCl2, 0.1mM KCl, 0.05mM CaCl2,0.05mM MgCl2, 0.5mM NaCl, 0.1mM Na2SO4, 0.3mM MES, and 0.1% sucrose, pH 5.7
  • 通讯作者:华中农业大学王荔军、刘建


The stresses acting on plants that are alleviated by silicon (Si) range from biotic to abiotic stresses, such as heavy metal toxicity. However, the mechanism of stress alleviation by Si at the single‐cell level is poorly understood.

We cultivated suspended rice (Oryza sativa) cells and protoplasts and investigated them using a combination of plant nutritional and physical techniques including inductively coupled plasma mass spectrometry (ICP‐MS), the scanning ion‐selective electrode technique (SIET) and X‐ray photoelectron spectroscopy (XPS).

We found that most Si accumulated in the cell walls in a wall‐bound organosilicon compound. Total cadmium (Cd) concentrations in protoplasts from Si‐accumulating (+Si) cells were significantly reduced at moderate concentrations of Cd in the culture medium compared with those from Si‐limiting (−Si) cells. In situ measurement of cellular fluxes of the cadmium ion (Cd2+) in suspension cells and root cells of rice exposed to Cd2+ and/or Si treatments showed that +Si cells significantly inhibited the net Cd2+ influx, compared with that in −Si cells. Furthermore, a net negative charge (charge density) within the +Si cell walls could be neutralized by an increase in the Cd2+ concentration in the measuring solution.

A mechanism of co‐deposition of Si and Cd in the cell walls via a [Si‐wall matrix]Cd co‐complexation may explain the inhibition of Cd ion uptake, and may offer a plausible explanation for the in vivo detoxification of Cd in rice.



我们培育了悬浮水稻(Oryza sativa)细胞和原生质体,并结合植物营养和物理技术进行了研究,包括电感耦合等离子体质谱(ICP-MS),扫描离子选择性电极技术(SIET)和X射线光电子。光谱学(XPS)。


通过[Si-壁基质] Cd共络合在细胞壁中共沉积Si和Cd的机制可以解释Cd离子吸收的抑制作用,并可能为水稻中Cd的体内解毒提供合理的解释。

Kinetics of cadmium (Cd2+) fluxes in a suspension-cultured cell in the presence of 30 lMCd2+ in the measuring medium determined using the scanning ion-selective electrode technique (SIET). Black,Si +Cd; red, +Si +Cd.