NMT历史上的今天丨New Phytol 纳米颗粒提升植物镉耐受性成果发表

转自中关村旭月非损伤微测技术产业联盟

NMT历史上的今天

2012年8月13日，华中农业大学王荔军、刘建利用NMT在Physiologia Plantarum上发表了标题为Inhibition of cadmium ion uptake in rice (Oryza sativa) cells by a wall-bound form of silicon的文章。

期刊：New Phytologist
主题：通过壁结合形式的硅抑制水稻（Oryza sativa）细胞中镉离子的吸收
标题：Inhibition of cadmium ion uptake in rice (Oryza sativa) cells by a wall-bound form of silicon
影响因子：6.736
检测指标：、Cd²⁺流速
检测部位：水稻根（距离根尖0、200、400、600、800、2000μm）、悬浮细胞
Cd²⁺流实验处理方法：一个月的水稻幼苗，0、1mM 硅酸处理2个月（由固体培养基转至液体培养基后）
Cd²⁺流实验测试液成份：0.03mM CdCl2, 0.1mM KCl, 0.05mM CaCl2,0.05mM MgCl2, 0.5mM NaCl, 0.1mM Na2SO4, 0.3mM MES, and 0.1% sucrose, pH 5.7
通讯作者：华中农业大学王荔军、刘建

英文摘要

The stresses acting on plants that are alleviated by silicon (Si) range from biotic to abiotic stresses, such as heavy metal toxicity. However, the mechanism of stress alleviation by Si at the single‐cell level is poorly understood.

We cultivated suspended rice (Oryza sativa) cells and protoplasts and investigated them using a combination of plant nutritional and physical techniques including inductively coupled plasma mass spectrometry (ICP‐MS), the scanning ion‐selective electrode technique (SIET) and X‐ray photoelectron spectroscopy (XPS).

We found that most Si accumulated in the cell walls in a wall‐bound organosilicon compound. Total cadmium (Cd) concentrations in protoplasts from Si‐accumulating (+Si) cells were significantly reduced at moderate concentrations of Cd in the culture medium compared with those from Si‐limiting (−Si) cells. In situ measurement of cellular fluxes of the cadmium ion (Cd²⁺) in suspension cells and root cells of rice exposed to Cd²⁺ and/or Si treatments showed that +Si cells significantly inhibited the net Cd²⁺ influx, compared with that in −Si cells. Furthermore, a net negative charge (charge density) within the +Si cell walls could be neutralized by an increase in the Cd²⁺ concentration in the measuring solution.

A mechanism of co‐deposition of Si and Cd in the cell walls via a [Si‐wall matrix]Cd co‐complexation may explain the inhibition of Cd ion uptake, and may offer a plausible explanation for the in vivo detoxification of Cd in rice.

中文摘要（谷歌机翻）

作用于植物的应力由硅（Si）缓解，范围从生物应力到非生物胁迫，例如重金属毒性。然而，人们对单细胞水平的Si应力缓解机制知之甚少。

我们培育了悬浮水稻（Oryza sativa）细胞和原生质体，并结合植物营养和物理技术进行了研究，包括电感耦合等离子体质谱（ICP-MS），扫描离子选择性电极技术（SIET）和X射线光电子。光谱学（XPS）。

我们发现大多数Si在壁结合的有机硅化合物中积聚在细胞壁中。与来自Si限制性（-Si）细胞的那些相比，培养基中中等浓度Cd下来自Si积累（+Si）细胞的原生质体中的总镉（Cd）浓度显着降低。原位测量暴露于Cd²⁺和/或Si处理的水稻悬浮细胞和根细胞中镉离子（Cd²⁺）的细胞通量表明+Si细胞显着抑制净Cd²⁺流入，与-Si细胞相比。此外，+Si电池壁内的净负电荷（电荷密度）可以通过测量溶液中Cd2+浓度的增加来中和。

通过[Si-壁基质] Cd共络合在细胞壁中共沉积Si和Cd的机制可以解释Cd离子吸收的抑制作用，并可能为水稻中Cd的体内解毒提供合理的解释。

Kinetics of cadmium (Cd²⁺) ﬂuxes in a suspension-cultured cell in the presence of 30 lMCd²⁺ in the measuring medium determined using the scanning ion-selective electrode technique (SIET). Black,Si +Cd; red, +Si +Cd.

文章链接：https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.12494

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