技术概况

技术简介

非损伤微测技术（Non-invasive Micro-test Technology, NMT）是实时测定进出活体材料离子和小分子流速的技术，是生理功能研究的最佳工具之一。

什么是NMT	三分钟看懂非损伤样品检测

活体乳腺肿瘤组织O₂、H⁺检测	原代神经元Ca²⁺流检测

视频开始播放后，建议点击暂停15秒左右，待视频缓冲后，播放更加流畅。

非损伤微测技术(NMT) 自从1974年美国海洋生物学实验室（MBL，Marine Biological Laboratory）的神经科学家Lionel F. Jaffe提出原初概念，到1990年成功应用于测定细胞的Ca²⁺流速，已经解决了众多科学问题。2001年，中国学者许越先生与Dr.Jaffe以美国扬格公司 (YoungerUSA, LLC) 为依托，进一步完善系统功能和用户体验，初步形成了现代NMT的雏形。2002－2005年，美国扬格公司以其优越的技术性能，为美国航空航天局某项目提供技术服务。2005年起，美国扬格公司与旭月（北京）科技有限公司展开全面战略合作，逐步完成了非损伤微测技术及系统的商业化，并且建立了完善的售后服务体系。随后，为了降低系统价格，美国扬格公司与旭月公司又联手推出了在中国组装的非损伤微测系统，继续不遗余力地推动非损伤微测技术在中国的应用和普及。

在非损伤微测技术的进一步发展和完善过程中，旭月公司获批的直接和相关技术专利已超过20项，形成了完善的知识产权保护体系，为非损伤微测技术在中国的未来发展奠定了法律基础。

NMT原理

非损伤微测技术是通过微电极和微传感器获取离子和分子的信号，基于Nernst方程和Fick's第一扩散定律计算离子和分子的浓度和流速，能够获得非常细微的信号，流速能够达到10^-12 mol • cm^-2 • s^-1。在生命科学领域，应用非常广泛，是连接生命功能的桥梁。在环境科学领域，非损伤微测技术的高灵敏度是人们探知环境恶化的预警系统。在材料科学领域，对于人们认识材料在液体环境中的性能提供了一个新颖的评价手段。

以Na⁺浓度梯度和Na⁺微电极为例说明非损伤微测技术离子选择性微电极的工作原理。

Na⁺离子选择性微电极通过前端灌充液态离子交换剂(Liquid Ion Exchanger，LIX)实现Na⁺的选择性。该微电极在待测离子浓度梯度中以已知距离dx进行两点测量，分别获得电压V₁和V₂。两点间的浓度差dc则可以从V₁、V₂及已知的该微电极的电压/浓度校正曲线(基于Nernst方程)计算获得。D是离子的扩散常数( 单位：cm²•sec^-1)，将它们代入Fick第一扩散定律公式J = - D • dc/dx，可获得该离子的流动速率(pico mol • cm^-2 • s^-1) 即：每秒钟通过每平方厘米的该离子/分子摩尔数（10^-12级）。

视频开始播放后，建议点击暂停15秒左右，待视频缓冲后，播放更加流畅。

注：荧光染料/光纤、纳米碳丝、酶电极、金属/合金等均可用来实现对某种离子/分子的选择性测量。

非损伤微测技术的测量有如下优势：

活体、原位、非损伤测定：生理功能研究，获取正在发生的信息
时间分辨率高：0.2—1s（响应时间），3.5s—18.5s（测量时间），能够快速获得细胞的早期事件
空间分辨率高：0.5 μm—10 μm，能够测定单细胞，甚至原生质体、液泡等细胞器
灵敏度高：10^-12～10^-15mol•cm^-2•s^-1
长时间测量：可进行长达几个小时，甚至更长时间的实时和动态监测
多种离子同时测量：研究离子间的相互关系
不用提取样品：直接测量，不需要研磨等传统的提取方法
动态实时测量：动态实时地测量和获取数据，可视化结果
操作便捷：很多操作通过自动控制可以实现
多个尺度上的测量：整体、器官、组织、细胞、甚至细胞器都可以测量（大于5μm）
立体3D流速测量：可在样品外进行X、Y、Z三维数据采集，清晰阐明样品及流速的空间相互关系

NMT应用基础知识

可测指标

目前旭月研究院能够测定的指标：Ca²⁺、H⁺、Na⁺、K⁺、Cl^-、Mg²⁺、Cd²⁺、Cu²⁺、Pb²⁺、NH₄⁺、NO₃^-、IAA、O₂、H₂O₂。

可测材料

1. 植物

（1）营养器官

根：根、根毛、根瘤

茎：边材、心材、微管形成层、木质部

叶：表皮细胞、叶肉细胞、盐腺细胞、保卫细胞

（2）生殖器官

花：花瓣、花瓣表皮细胞、花粉

种子：整体、胚

果实：果壳、果皮、果肉（苹果、柑橘）、籽粒、棉花纤维、棉桃

（3）细胞：植物悬浮细胞、液泡

（4）愈伤组织

2. 动物

（1）细胞

神经细胞、肿瘤细胞、巨噬细胞、淋巴细胞等

（2）组织器官

肿瘤、皮肤、胃粘膜、胰岛、脑（海马体等）、胚胎（大鼠、鱼）、耳蜗、心脏（香螺）、卵（鱼、鸡蛋、爪蟾）、骨骼、角膜、脊椎（豚鼠）、肌肉组织（肌纤维、心肌）

（3）其它生物种类

珊瑚、螨虫、昆虫（果蝇幼虫的肠、蟑螂血脑屏障、按蚊、长红锥蝽）、蝌蚪、水蛭、蓝蟹（微感毛）、变形虫

3. 微生物

酵母细胞、菌丝、微藻、细菌（白腐真菌、大肠杆菌）

4. 非生物材料

合金、泥沙、纳米复合材料

可研究领域

医学

1. 总括——NMT医学生理学研究覆盖方向

 2. 肿瘤研究

 3. 神经研究

 4. 消化系统研究

 5. 骨骼研究

 6. 代谢疾病研究

 7. 免疫（淋巴细胞）研究

 8. 细胞凋亡

 9. 药理学研究

 10. 模式动物（斑马鱼）研究

 11. 农药毒理研究思路

植物科学

1. 总括——您的研究与哪些离子/分子相关

2. 植物逆境

3. 植物营养

4. 作物育种

抗盐育种

5. 光合研究

6. 植物微生物互作

7. 植物防御

8. 生长发育

精选文献

医学动物科学

细胞凋亡

C2015-014，李成章，武汉大学口腔医学院，PHOTOMED LASER SURG，口腔鳞状癌细胞，O₂/Ca²⁺

O2 and Ca2+ Fluxes as Indicators of Apoptosis Induced by Rose Bengal–Mediated Photodynamic Therapy in Human Oral Squamous Carcinoma Cells

C2014-002，冯华，第三军医大学附属西南医院神经外科，Lasers in Medical Science，神经胶质瘤细胞，Ca2+/K+

Imbalance of Ca2+ and K+ fluxes in C6 glioma cells after PDT measured with scanning ion-selective electrode technique

F2011-005，Nature Cell Biology，神经元，O2

Bcl xL regulates metabolic ef ciency of neurons through interaction with the mitochondrial F1F0 ATP synthas

F2010-005，Journal of Cell Science，拟南芥根，K+

Arabidopsis root K+-efflux conductance activated by hydroxyl radicals， single-channel properties, genetic basis and involvement in stress-induced cell death

F2000-010，Biology of Reproduction，小鼠胚胎，O2

Oxidative Phosphorylation-Dependent and -Independent Oxygen Consumption by Individual Preimplantation Mouse Embryos

F2009-008，AM J PHYSIOL-CELL PH，T淋巴细胞，K+

Kbg and Kv1.3 channels mediate potassium efflux in the early phase of apoptosis in Jurkat T lymphocytes

肿瘤研究

F2018-005，BBA-GEN SUBJECTS，黑色素瘤细胞，非肿瘤黑素细胞，H+

Tumor cell cholesterol depletion and V-ATPase inhibition as an inhibitory mechanism to prevent cell migration and invasiveness in melanoma

C2015-014，李成章，武汉大学口腔医学院，PHOTOMED LASER SURG，口腔鳞状癌细胞，O2/Ca2+

O2 and Ca2+ Fluxes as Indicators of Apoptosis Induced by Rose Bengal–Mediated Photodynamic Therapy in Human Oral Squamous Carcinoma Cells

C2014-002，冯华，第三军医大学附属西南医院神经外科，Lasers in Medical Science，神经胶质瘤细胞，Ca2+/K+

Imbalance of Ca2+ and K+ fluxes in C6 glioma cells after PDT measured with scanning ion-selective electrode technique

C2012-018，王立伟，暨南大学，Biochemical Pharmacology，鼻咽癌细胞，K+/Cl-/H+

Uncoupling of K+ and Cl- transport across the cell membrane in the process of regulatory volume decrease

F2012-007，Cell biochemistry and Function，结肠癌细胞，Ca2+/K+

Exposure of colonic epithelial cells to oxidative and endoplasmic reticulum stress causes rapid potassium efflux and calcium influx

C2008-001，许越，旭月（北京）科技有限公司，生物物理学报，乳腺癌细胞，H+

用非损伤微测技术研究肿瘤细胞的耐药性与其胞外H+流变化的相关性

活体肿瘤H+/O2流速同癌占比具有相关性

神经生物学

H2017-001，祁金顺，山西医科大学，生理学报，脑片，Ca2+

非损伤微测技术实时检测海马脑片跨膜钙离子流

C2014-002，冯华，第三军医大学附属西南医院神经外科，Lasers in Medical Science，神经胶质瘤细胞，Ca2+/K+

Imbalance of Ca2+ and K+ fluxes in C6 glioma cells after PDT measured with scanning ion-selective electrode technique

F2012-005，Brain Research，神经元，O2

Effects of dexpramipexole on brain mitochondrial conductances and cellular bioenergetic efficiency

F2011-019，PLoS One，神经元，H+/K+

Tg2576 Cortical Neurons That Express Human Ab Are Susceptible to Extracellular Aβ-Induced, K+ Efflux Dependent Neurodegeneration

F2011-005，Nature Cell Biology，神经元，O2

Bcl xL regulates metabolic ef ciency of neurons through interaction with the mitochondrial F1F0 ATP synthas

F2010-017，Molecular Neurodegeneration，神经元，Ca2+/K+

Prolonged Aβ treatment leads to impairment in the ability of primary cortical neurons to maintain K+ and Ca2+ homeostasis

F2010-021，Plos One，神经元，Ca2+/K+

The Native Copper- and Zinc- Binding Protein Metallothionein Blocks Copper-Mediated Aβ Aggregation and Toxicity in Rat Cortical Neurons

F2010-001，J NEUROSCI METH，神经元，葡萄糖

A self-referencing glutamate biosensor for measuring real time neuronal glutamate flux

F2009-018，Journal of Neurochemistry，神经元，O2

Simultaneous single neuron recording of O2 consumption,Ca2+ and mitochondrial membrane potential in glutamate toxicity

F2008-014，J BIOL ENG，脊髓，Ca2+

Large naturally-produced electric currents and voltage traverse damaged mammalian spinal cord

F2005-020，AM J PHYSIOL-CELL PH，神经胶质细胞，H2O2

Synergistic amplification of beta-amyloid- and interferon-gamma-induced microglial neurotoxic response by the senile plaque component chromogranin A.

代谢疾病

C2016-014，沈应柏，北京林业大学，PLoS One，肝组织，葡萄糖

Leaf Extract from Lithocarpus polystachyus Rehd. Promote Glycogen Synthesis in T2DM Mice

C2012-011，张宗明，清华大学，Chinese Medical Journal，胰岛细胞，Ca2+

Abnormal mitochondrial function impairs calcium influx in diabetic mouse pancreatic beta cells

F2011-024，Analytical Biochemistry，胰岛细胞，葡萄糖

Oscillatory glucose flux in INS 1 pancreatic β cells: A selfreferencing microbiosensor study

F2009-015，AM J PHYSIOL-ENDOC，胰岛细胞，O2

Role for malic enzyme pyruvate carboxylation and mitochondrial malate import in the glucose-stimulated insulin secretion

F2005-016，Diabetes，肌肉细胞，O2

Physiological Increases in Uncoupling Protein Augment Fatty Acid Oxidation and Decrease Reactive Oxygen Species 0Production Without Uncoupling Respiration in Muscle Cells

F2000-011，Diabetes，胰岛细胞，O2

Oxygen consumption oscillates in single clonal pancreatic beta -cells (HIT)

骨骼研究

F2018-003，BONE，骨细胞，Ca2+

Calcium fluxes at the bone/plasma interface: acute effects of parathyroid hormone (PTH) and targeted deletion of PTH/PTHrelated peptide (PTHrP) receptor in the osteocytes

F2012-003，BONE，骨组织，Ca2+

Mechanical Stretch Induced Calcium Efflux from Bone Matrix Stimulates Osteoblasts

F2005-003，BONE，骨组织，Ca2+

Bone as an ion exchange organ Evidence for instantaneous cell-dependent calcium efflux from bone not due to resorption

消化生理

C2015-012，朱进霞，首都医科大学，WORLD J GASTROENTERO，结肠粘膜，Cl-

Effect of entacapone on colon motility and ion transport in a rat model of Parkinson’s disease

C2012-008，张宗明，清华大学，WORLD J GASTROENTERO，肝脏细胞，Ca2+

Effects and mechanisms of store-operated calcium channel blockade on hepatic ischemia-reperfusion injury in rats

C2012-007，朱进霞，首都医科大学，BRIT J PHARMACOL，结肠粘膜，Cl-

Emodin induces chloride secretion in rat distal colon through activation of mast cells and enteric neurons

C2011-004，朱进霞，首都医科大学，NEUROGASTROENT MOTIL，肠粘膜，Cl-

Entacapone promotes cAMP-dependent colonic Cl- secretion in rats

C2009-001，张宗明，清华大学，WORLD J GASTROENTERO，肝脏细胞，Ca2+

Measuring Ca2+ influxes of TRPC1-dependent Ca2+ channels in HL-7702 cells with Non-invasive Micro-test Technique

药理学

C2012-007，朱进霞，首都医科大学，BRIT J PHARMACOL，结肠粘膜，Cl-

Emodin induces chloride secretion in rat distal colon through activation of mast cells and enteric neurons

C2011-004，朱进霞，首都医科大学，NEUROGASTROENT MOTIL，肠粘膜，Cl-

Entacapone promotes cAMP-dependent colonic Cl- secretion in rats

再生医学

F2014-008，Integrative Biology，爪蟾卵母细胞，Ca2+

Single cell wound generates electric current circuit and cell membrane potential variations that requires calcium influx

F2011-011，PLoS One，角膜，Ca2+/K+/Na+/Cl-

Ionic Components of Electric Current at Rat Corneal Wounds

模式生物

F2018-004，J EXP BIOL，果蝇马氏管、肠组织，K+

Cold tolerance of Drosophila species is tightly linked to epithelial K+ transport capacity of the Malpighian tubules and rectal pads

F2018-002，J EXP BIOL，果蝇马氏管、肠组织，K+

Functional Plasticity of the gut and the Malpighian tubules underlies cold acclimation and mitigates cold-induced hyperkalemia in Drosophila melanogaster

F2017-001，J INSECT PHYSIOL，果蝇肠上皮细胞，H+/K+

The roles of V-type H+-ATPase and Na+/K+-ATPase in energizing K+ and H+ transport in larval Drosophila gut epithelia

F2016-003，J INSECT PHYSIOL，果蝇马氏管，Ca2+

Segment-specific Ca(2+) transport by isolated Malpighian tubules of Drosophila melanogaster: A comparison of larval and adult stages

F2014-004，ARCH INSECT BIOCHE，果蝇肠组织，K+

Effects of diuretic hormone 31,drosokinin,and allatostatin a on transepithelial K+ Transport and contraction frequencty in the midgut and hindgut of larval

F2013-007，J EXP BIOL，果蝇马氏管，NH4+/K+

Ammonium secretion by Malpighian tubules of Drosophila melanogaster: application of a novel ammonium-selective microelectrode

植物科学

盐碱胁迫

C2018-009，别之龙/黄远，华中农业大学，J EXP BOT，叶脉/叶片/根，Na+/K+

Salt tolerance mechanisms in pumpkin species (Cucurbita)

C2017-026，别之龙，华中农业大学，J EXP BOT，根/下胚轴，Na+/H+

Root respiratory burst oxidase homologue-dependent H2O2 production confers salt tolerance on a grafted cucumber by controlling Na+ exclusion and stomatal closure

C2016-003，张明才，中国农业大学，J EXP BOT，根，Na+/K+/H+

Increased abscisic acid levels in transgenic maize overexpressing AtLOS5 mediated root ion fluxes and leaf water status under salt stress

C2016-002，柳参奎，东北林业大学，PLANT BIOTECHNOL J，根，Na+

Conserved V-ATPase c subunit plays a role in plant growth by influencing V-ATPase-dependent endosomal trafficking

C2015-032，郑海雷，厦门大学，Scientific Reports，根，Na+/K+

Hydrogen sulfide enhances salt tolerance through nitric oxide mediated maintenance of ion homeostasis in barley seedling roots

C2015-030，张洪霞，中科院上海植物生理生态研究所，PLANT BIOTECHNOL J，根，Na+

Overexpression of the PtSOS2 gene improves tolerance to salt stress in transgenic poplar plants

C2015-005，种康，中科院植物所，Cell，根，Ca2+

COLD1 Confers Chilling Tolerance in Rice

F2015-008，PLANT CELL ENVIRON ，黄藻，H+/K+ Na+

Mechanisms underlying turgor regulation in the estuarine alga Vaucheria erythrospora (Xanthophyceae) exposed to hyperosmotic shock

F2014-006，PLANT CELL ENVIRON ，根木质部，K+

Kinetics of xylem loading， membrane potential maintenance，and sensitivity of K+-permeable channels to reactive oxygen species: physiological traits that differentiate salinity tolerance between pea and barley

C2013-021，余迪求，中科院西双版纳热带植物园，Plant Journal，根，K+

Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance

C2012-020，魏建华，北京市农林科学院，PLANT CELL ENVIRON ，根，K+/H+

HbCIPK2, a novel CBL-interacting protein kinase from halophyte Hordeum brevisubulatum, confers salt and osmotic stress tolerance

C2012-010，陈少良，北京林业大学，Plant Physiology，根尖，Na+/K+/H+/Ca2+

Paxillus involutus strains MAJ and NAU mediate K+/Na+ homeostasis in ectomycorrhizal Populus × canescens under NaCl stress

C2010-004，陈少良，北京林业大学，PLANT CELL ENVIRON ，愈伤组织细胞，Na+/K+/H+/Ca2+

H2O2 and cytosolic Ca2+ signals triggered by the PM H+-coupled transport system mediate K+/Na+ homeostasis in NaCl-stressed Populus euphratica cells

C2010-002，郭岩，北京生命科学研究所，Plant Cell，根，H+

The Arabidopsis chaperone J3 regulates the plasma membrane H+-ATPase through interaction with the PKS5 kinase

C2009-004，陈少良，北京林业大学，Plant Physiology，根，Na+/Cl-/H+

NaCl-induced alternations of cellular and tissue ion fluxes in roots of salt-resistant and salt-sensitive poplar species

温度胁迫

C2016-001，陈少良，北京林业大学，PLANT CELL TISS ORG，悬浮细胞，Ca2+/K+

Heat shock responses in Populus euphratica cell cultures important role of crosstalk among hydrogen peroxide, calcium and potassium

C2015-005，种康，中科院植物所，Cell，根，Ca2+

COLD1 Confers Chilling Tolerance in Rice

重金属胁迫

C2017-034，李芳柏，广东省科学院生态环境研究所，Environmental Pollution，悬浮细胞，Cd2+

Silica nanoparticles alleviate cadmium toxicity in rice cells: Mechanisms and size effects

C2015-003，王荔军，华中农业大学，New Phytologist，悬浮细胞，Cd2+

A hemicellulose-bound form of silicon inhibits cadmium ion uptake in rice (Oryza sativa) cells

C2015-001，罗志斌，西北农林科技大学，New Phytologist，根，Ca2+/Cd2+/H+

Overexpression of bacterial gamma-glutamylcysteine synthetase mediates changes in cadmium influx, allocation and detoxification in poplar

C2013-019，王荔军，华中农业大学，New Phytologist，悬浮细胞，Cd2+

Inhibition of cadmium ion uptake in rice (Oryza sativa) cells by a wall-bound form of silicon

C2013-015，罗志斌，西北农林科技大学，PLANT CELL ENVIRON ，根，Cd2+/H+

Ectomycorrhizas with Paxillus involutus enhance cadmium uptake and tolerance in Populus × canescens

C2012-017，徐进，中科院遗传与发育生物学研究所农业资源研究中心，New Phytologist，根，Cd2+

Comparative physiological responses of Solanum nigrum and Solanum torvum to cadmium stress

C2012-016，徐进，中科院遗传与发育生物学研究所农业资源研究中心，New Phytologist，根，Cd2+

Comparative transcriptome analysis of cadmium responses in Solanum nigrum and Solanum torvum

C2012-009，赵福庚，南京大学，PLANT CELL ENVIRON ，根，K+/Ca2+

Cadmium impairs ion homeostasis by altering K+ and Ca2+ channel activities in rice root hair cells

水旱胁迫

C2018-001，许卫锋/张建华，福建农林大学/香港中文大学，Plant Physiology，根，H+/Ca2+/H2O2

Comparative analysis of Arabidopsis 26 ecotypes reveals a role for brassinosteroids in root hydrotropism

C2015-023，许卫锋/张建华，中科院南京土壤研究所/香港中文大学，J EXP BOT，根，H+

Involvement of 14-3-3 protein GRF9 in root growth and response under PEG-induced water stress

F2014-012，ENVIRON EXP BOT，叶肉细胞，K+/H+/Ca2+

Leaf mesophyll K+， H+ and Ca2+ fluxes are involved in drought-induced decrease in photosynthesis and stomatal closure in soybean

C2013-006，施卫明/张建华，中科院南京土壤研究所，New Phytologist，根，H+

Abscisic acid accumulation modulates auxin transport in the root tip to enhance proton secretion for maintaining root growth under moderate water stress

C2013-004，陈保冬，中科院生态环境中心，New Phytologist，酵母细胞， Ca2+/H+

First cloning and characterization of two functional aquaporin genes from an arbuscular mycorrhizal fungus Glomus intraradices

F2013-009，Frontiers in Plant Science，大麦，H+/ K+

Barley responses to combined waterlogging and salinity stress:separating effects of oxygen deprivation and elemental toxicity

F2007-006，Plant Physiology，根，Ca2+/H+/K+

Effect of Secondary Metabolites Associated with Anaerobic Soil Conditions on Ion Fluxes and Electrophysiology in Barley Roots

F2005-008，Plant Journal，根，H+

Ethylene regulates fast apoplastic acidification and expansin a transcription during submergence-induced petiole elongation in rumex palustris

生物胁迫

C2017-033，王国梁/王毅，中国农科院/中国农业大学，Plos Pathogens，根，K+

The fungal pathogen Magnaporthe oryzae suppresses innate immunity by modulating a host potassium channel

C2017-018，沈应柏，北京林业大学，Functional Plant Biology，-，-

Plant ion channels and transporters in herbivory-induced signalling

C2017-003，沈应柏，北京林业大学，J PLANT GROWTH REGUL，根，IAA/H+

Insect Herbivory of Leaves Affects the Auxin Flux Along Root Apices in Arabidopsis thaliana

F2011-014，PLANT CELL ENVIRON ，叶肉细胞，Ca2+/ K+

Plasma membrane Ca2+ transporters mediate virus-induced acquired resistance to oxidative stress

F2010-013，Planta，叶肉细胞，K+/Ca2+/H+

Non-invasive microelectrode potassium flux measurements as a potential tool for early recognition of virus–host compatibility in plants

F2008-004，Plant Cell Physiology，叶肉细胞，Ca2+

Calcium Efflux as a Component of the Hypersensitive Response of Nicotiana benthamiana to Pseudomonas syringae

F2007-008，Planta，叶肉细胞，K+/H+

Expression of animal CED-9 anti-apoptotic gene in tobacco modifies plasma membrane ion fluxes in response to salinity and oxidative stress

植物营养

C2017-032，王毅，中国农业大学，Plant Cell，爪蟾卵母细胞，K+/H+

NRT1.5/NPF7.3 Functions as a Proton-Coupled H+/K+ Antiporter for K+ Loading into the Xylem in Arabidopsis

C2017-010，许卫锋/张建华，福建农林大学/香港中文大学，J EXP BOT，根，H+

Arabidopsis plasma membrane H+-ATPase genes AHA2 and AHA7 have distinct and overlapping roles in the modulation of root tip H+ efflux in response to low-phosphorus stress

C2016-004，张振华，湖南农业大学，Plant Physiology，液泡，NO3-

Nitrogen Use Efficiency Is Mediated by Vacuolar Nitrate Sequestration Capacity in Roots of Brassica napus

C2015-036，童依平，中科院遗传发育所，Plant Physiology，根，NO3-

The Nitrate-Inducible NAC Transcription Factor TaNAC2-5A Controls Nitrate Response and Increases Wheat Yield

C2015-007，童依平，中科院遗传与发育生物学研究所，Plant Physiology，根，NO3-

A wheat CCAAT box-binding transcription factor increases the grain yield of wheat with less fertilizer input

C2014-022，余玲，南京农业大学，Plant Physiology，根，K+

The Role of a Potassium Transporter OsHAK5 in Potassium Acquisition and Transport from Roots to Shoots in Rice at Low Potassium Supply Levels

C2013-020，罗志斌，西北农林科技大学，J EXP BOT，根，NH4+/NO3-/H+

Nitrogen metabolism of two contrasting poplar species during acclimation to limiting nitrogen availability

C2013-008，夏新莉/尹伟伦，北京林业大学，PLANT CELL ENVIRON ，根，NO3-

The nitrate transporter NRT2.1 functions in the ethylene response to nitrate deficiency in Arabidopsis

C2012-021，徐国华，南京农业大学，Plant Physiology，根，NO3-

Knockdown of a rice stelar nitrate transporter alters long-distance translocation but not root influx

C2012-006，施卫明/张建华，中科院南京土壤研究所，PLANT CELL ENVIRON ，根，H+

TFT6 and TFT7, two different members of tomato 14-3-3 gene family, play distinct roles in plant adaption to low phosphorus stress

C2010-006，施卫明，中科院南京土壤研究所，PLANT CELL ENVIRON ，根，NH4+

Root growth inhibition by NH4+ in Arabidopsis is mediated by the root tip and is linked to NH4+ efflux and GMPase activity

生长发育

F2018-001，Science，花粉管，Ca2+

CORNICHON sorting and regulation of GLR channels underlie pollen tube Ca2+ homeostasis

F2016-001，Plant Cell，根，IAA

TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton dynamics

C2014-010，孙蒙祥，武汉大学，J EXP BOT，花粉管，Ca2+

Exogenous γ-aminobutyric acid affects pollen tube growth via modulating putative Ca2+-permeable membrane channels and is coupled to negative regulation on glutamate decarboxylase

C2014-006，宋纯鹏，河南大学，Plant Cell，根毛/液泡，NH4+/Ca2+

A Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in Arabidopsis

C2014-004，涂礼莉，华中农业大学，PLANT BIOTECHNOL J，纤维细胞，K+

A peptide hormone gene, GhPSK promotes fibre elongation and contributes to longer and finer cotton fibre

C2014-003，张献龙/涂礼莉，华中农业大学，New Phytologist，纤维细胞，Ca2+

The calcium sensor GhCaM7 promotes cotton fiber elongation by modulating reactive oxygen species (ROS) production

F2013-008，Plant Cell，根，IAA

Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter ABCB1 on the Root Plasma Membrane

C2012-013，林金星，中科院植物研究所，Plant Cell，根，IAA

The Signal Transducer NPH3 Integrates the Phototropin1 Photosensor with PIN2-Based Polar Auxin Transport in Arabidopsis Root Phototropism

F2011-020，J EXP BOT，花粉管，Cl-

The essential role of anionic transport in plant cells: the pollen tube as a case study

F2011-009，Science，花粉管，Ca2+

Glutamate Receptor–Like Genes Form Ca2+ Channels in Pollen Tubes and Are Regulated by Pistil D-Serine

C2009-007，林金星，中科院植物研究所，Plant Physiology，花粉管，Ca2+

Combined proteomic and cytological analysis of Ca2+-calmodulin regulation in Picea meyeri pollen tube growth

C2009-003，林金星，中科院植物研究所，J BIOL CHEM，原生质体，Ca2+

Calmodulin binds to extracellular sites on the plasma membrane of plant cells and elicits a rise in intracellular calcium concentration

C2009-002，林金星，中科院植物研究所，New Phytologist，花粉管，Ca2+

Nitric oxide modulates the influx of extracellular Ca2+ and actin filament organization during cell wall construction in Pinus bungeana pollen tubes

F2007-014，PNAS，根毛，H+

Oscillations in extracellular pH and reactive oxygen species modulate tip growth of Arabidopsis root hairs

F2002-001，PNAS，根，H+/Ca2+

Blue light-induced kinetics of H+ and Ca2+ fluxes in etiolated wild-type and phototropin-mutant Arabidopsis seedlings

F2001-004，Nature Cell Biology，玉米卵细胞，Ca2+

Differential contribution of cytoplasmic Ca2+and Ca2+ influx to gamete fusion and egg activation in maize

F2000-001，PNAS，玉米卵细胞，Ca2+

A calcium influx is triggered and propagates in the zygote as a wavefront during in vitro fertilization of flowering plants

互作共生

C2017-002，Frontiers in Plant Science，陈少良，北京林业大学，菌根，H+/Ca2+/Cd2+/H2O2

Paxillus involutus-Facilitated Cd2+ Influx through Plasma Membrane Ca2+-Permeable Channels Is Stimulated by H2O2 and H+-ATPase in Ectomycorrhizal Populus × canescens under Cadmium Stress

F2015-009，Molecular Ecology，菌根，H+/NH4+/NO3-

Species turnover (β diversity) in ectomycorrhizal fungi linked to NH4 (+) uptake capacity

C2014-021，ENVIRON EXP BOT，陈少良，北京林业大学，根，H+/Na+/K+/Ca2+

Ion fluxes in Paxillus involutus-inoculated roots of Populus × canescensunder saline stress

C2014-012，Mycorrhiza，吴强盛，长江大学，根，H2O2/ Ca2+

Mycorrhiza-induced lower oxidative burst is related with higher antioxidant enzyme activities, net H2O2 effluxes, and Ca2+ influxes in trifoliate orange roots under drought stress

C2013-015，PLANT CELL ENVIRON ，罗志斌，西北农林科技大学，根，Cd2+/H+

Ectomycorrhizas with Paxillus involutus enhance cadmium uptake and tolerance in Populus × canescens

C2012-010，Plant Physiology，陈少良，北京林业大学，根/真菌团，Na+/K+/H+/Ca2+

Paxillus involutus strains MAJ and NAU mediate K+/Na+ homeostasis in ectomycorrhizal Populus × canescens under NaCl stress

F2009-001，New Phytologist，根，H+

A pH signaling mechanism involved in the spatial distribution of calcium and anion fluxes in ectomycorrhizal roots

F2008-019，New Phytologist，丛枝菌根真菌，H+

Proton (H+) flux signature for the presymbiotic development of the arbuscular mycorrhizal fungi

F2008-018，Plant Signaling & Behavior，丛枝菌根真菌，H+

pH signature for the responses of arbuscular mycorrhizal fungi to external stimuli

微生物学

C2018-013，李哲/肖艳，中科院重庆绿色智能技术研究院，Frontiers in Plant Science，微藻，O2

Microalgae in Microwell Arrays Exhibit Differences with Those in Flasks: Evidence from Growth Rate, Cellular Carotenoid, and Oxygen Production

C2017-002，陈少良，北京林业大学，Frontiers in Plant Science，菌根，H+/Ca2+/Cd2+/H2O2

Paxillus involutus-Facilitated Cd2+ Influx through Plasma Membrane Ca2+-Permeable Channels Is Stimulated by H2O2 and H+-ATPase in Ectomycorrhizal Populus × canescens under Cadmium Stress

C2017-028，尹华，华南理工大学，Environmental Pollution，真菌，K+

Hexavalent chromium induced oxidative stress and apoptosis in Pycnoporus sanguineus

C2016-020，夏传海，中科院烟台海岸带研究所，Marine Pollution Bulletin，细柱藻，Ca2+

The possible role of bacterial signal molecules N-acyl homoserine lactones in the formation of diatom-biofilm (Cylindrotheca sp.)

C2015-004，曾光明/陈桂秋，湖南大学，Chemosphere，黄孢原毛平革菌，H+/ O2/ Cd2+

Physiological fluxes and antioxidative enzymes activities of immobilized Phanerochaete chrysosporium loaded with TiO2 nanoparticles after exposure to toxic pollutants in solution

F2015-004，PLANT CELL ENVIRON ，微藻，H+/ K+/Na+

Mechanisms underlying turgor regulation in the estuarine alga Vaucheria erythrospora (Xanthophyceae) exposed to hyperosmotic shock

C2014-007，王强，中科院水生所，Plant and Cell Physiology，绿藻，Ca2+

Ca2+ Signal Transduction Related to Neutral Lipid Synthesis in an Oil-Producing Green Alga Chlorella sp. C2

C2012-014，曾光明/陈桂秋，湖南大学，ENVIRON SCI TECHNO，白腐真菌，H+/ O2/ Cd2+

Responses of Phanerochaete chrysosporium to Toxic Pollutants: Physiological Flux, Oxidative Stress, and Detoxification

F2012-009，Planta，布拉克须霉，Ca2+/ H+

Surface tip-to-base Ca2+ and H+ ionic fluxes are involved in apical growth and graviperception of the Phycomyces stage I sporangiophore

F2012-006，Eukaryotic Cell，链孢霉，Ca2+

Electrical Phenotypes of Calcium Transport Mutant Strains of a Filamentous Fungus, Neurospora crassa

F2009-012，Environmental Microbiology，破囊壶菌，Na+/K+/Cl-/Ca2+

Osmotic adjustment and requirement for sodium in marine protist thraustochytrid

F2009-007，Environmental Microbiology，大肠杆菌，K+

Ion transport and osmotic adjustment in Escherichia coli in response to ionic and non-ionic osmotica

F2008-008，Research in Microbiology，大肠杆菌，H+

Cyclopropane fatty acids improve Escherichia coli survival in acidified minimal media by reducing membrane permeability to H+ and enhanced ability to extrude H+

F2007-020，Microbiology，脉胞菌，K+/ H+/Ca2+/Cl-

Turgor regulation in the osmosensitive cut mutant of Neurospora crassa

F2007-009，J EXP BOT，脉胞菌菌丝，K+/ H+/Ca2+/Cl-

Ionic currents and ion fluxes in Neurospora crassa hyphae

环境科学

C2017-028，尹华，华南理工大学，Environmental Pollution，真菌，K+

Hexavalent chromium induced oxidative stress and apoptosis in Pycnoporus sanguineus

C2015-004，曾光明/陈桂秋，湖南大学，Chemosphere，黄孢原毛平革菌，H+/ O2/ Cd2+

Physiological fluxes and antioxidative enzymes activities of immobilized Phanerochaete chrysosporium loaded with TiO2 nanoparticles after exposure to toxic pollutants in solution

C2014-008，郭仰东，中国农业大学，Plant and Cell Physiology，根/爪蟾卵母细胞，K+/H+

Brassica oleracea MATE Encodes a Citrate Transporter, and Enhances Aluminum Tolerance in Arabidopsis thaliana

C2012-014，曾光明/陈桂秋，湖南大学，ENVIRON SCI TECHNO，白腐真菌，H+/ O2/ Cd2+

Responses of Phanerochaete chrysosporium to Toxic Pollutants: Physiological Flux, Oxidative Stress, and Detoxification

C2012-004，朱永官，中科院生态环境中心，Bioresource Technology，水稻根，O2

A novel sediment microbial fuel cell with a biocathode in the rice rhizosphere

F2010-012，ENVIRON SCI TECHNO，生物膜，H+/O2

Membrane-Aerated Biofilm Proton and Oxygen Flux during Chemical Toxin Exposure Running Title Toxicity and Stress Response in Membrane Aerated Biofilms

F2008-017，ENVIRON SCI TECHNO，鱼胚胎，O2

Oxygen Flux As an Indicator of Physiological Stress in Fathead Minnow (Pimephales promelas) Embryos A Real-Time Biomonitoring System of Water Quality

水产研究

F2017-018，Scientific Reports，青鳉皮肤离子细胞，K+

Potassium Regulation in Medaka (Oryzias latipes) Larvae Acclimated to Fresh Water: Passive Uptake and Active Secretion by the Skin Cells

F2009-006，AM J PHYSIOL-CELL PH，斑马鱼皮肤离子细胞，H+

Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment

F2015-002，PLoS ONE，斑马鱼皮肤离子细胞，H+

Aquaporin 1 Is Involved in Acid Secretion by Ionocytes of Zebrafish Embryos through Facilitating CO2 Transport

F2015-001，INT J BIOL SCI，斑马鱼皮肤离子细胞，H+/ NH4+

Induction of Phosphoenolpyruvate Carboxykinase(PEPCK) during Acute Acidosis and Its Role in Acid Secretion by V-ATPase-Expressing Ionocytes

F2013-017，Am J Physiol Regul Integr Comp Physiol，青鳉鱼皮肤离子细胞，H+/NH4+

Proton-facilitated ammonia excretion by ionocytes of medaka (Oryzias latipes)acclimated to seawater

F2012-011，Am J Physiol Regul Integr Comp Physiol.，青鳉鱼皮肤离子细胞，H+/ Na+/NH4+

Acid secretion by mitochondrion-rich cells of medaka (Oryzias latipes) acclimated to acidic freshwater

昆虫生理

F2018-007，ENVIRON TOXICOL CHEM，摇蚊肠组织/马氏管/肛乳突，Cd2+/Ca2+

Characterization of Cadmium and Calcium Fluxes Along the Gut, Malpighian Tubules and Anal Papillae of the Dipteran, Chironomus riparius

F2018-006，J INSECT PHYSIOL，粉纹夜蛾马氏管，K+/Na+

Molecular mechanisms of bi-directional ion transport in the Malpighian tubules of a lepidopteran crop pest, Trichoplusia ni

F2016-004，J EXP BIOL，埃及伊蚊肛乳突，NH4+

An animal homolog of plant Mep/Amt transporters promotes ammonia excretion by the anal papillae of the disease vector mosquito Aedes aegypti.

F2015-006，J EXP BIOL，甘蓝夜蛾直肠/马氏管，K+/Na+

The rectal complex and Malpighian tubules of the cabbage looper (Trichoplusia ni): regional variations in Na+ and K+ transport and cation reabsorption by secondary cells

F2014-002，J EXP BIOL，东亚飞蝗回肠，K+

K+ absorption by locust gut and inhibition of ileal K+ and water transport by FGLamide allatostatins

F2014-001，PLoS ONE，埃及伊蚊后肠，Na+/K+

The Heterodimeric Glycoprotein Hormone, GPA2GPB5, Regulates Ion Transport across the Hindgut of the Adult Mosquito, Aedes aegypti

F2013-020，J INSECT PHYSIOL，埃及伊蚊中肠，H+/ K+/Na+

Transport of H+, Na+ and K+ across the posterior midgut of blood-fed mosquitoes (Aedes aegypti)