Bio-Monitoring of Environmental Pollutions
- Water pollution detection
- Biological early warning
- Water Quality Assessment
- Detection of heavy metal pollution
O2 and H+ activities are essential to all organisms, while aquatic organisms seem to be more sensitive than others in response to environmental changes. They are especially sensitive to pollutants making O2 and H+ fluxes good and reliable indicators of environmental pollutions.
It can be applied to water quality assessment, biological early warning of environmental pollutions.
O2 fluxes changes of fathead embryo in response to different pollutants
Sanchez BC, et al. Environ. Sci. Technol. 2008, 42: 7010-7017.
(Use with permission)
Single-cell, real-time measurements of extracellular oxygen and proton fluxes from Spirogyra grevilleana
||NMT is sensitive enough to measure oxygen fluxes around single cells. Porterfield and Smith et al. have characterized oxygen and proton fluxes from a single cell of the filamentous green alga Spirogyra gre illeana (Hass.).
|Oxygen showed a net efflux and protons showed a net influx when the cell was illuminated. These photosynthesis-dependent fluxes were found to be spatially associated with the chloroplasts and were sensitive to treatment with dichlorophenyldimethylurea. In the dark the directions of oxygen and proton fuxes were reversed. This oxygen influx was associated with mitochondrial respiration and was reduced by 78% when the cells was treated with 0.5 mM KCN. The residual cyanide-resistant respiration was inhibited by the application of 5 mM salicylhydroxamic acid, an inhibitor of the alternative oxidase. Similarly the cytochrome pathway was also inhibited by the presence of 20 uM NO, while the cyanide-resistant alternative oxidase was not.
These results demonstrate the use of the NMT system to measure and characterize metabolic fluxes at a level of sensitivity that allows for subcellular resolution.