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PBJ IAA流新成果:农科院棉花所、浙江农科院丨AKR2A协调IAA和H2O2积累调控棉纤维伸长

转自中关村旭月非损伤微测技术产业联盟


期刊:Plant Biotechnology Journal
主题:AKR2A协调IAA和H2O2积累调控棉纤维伸长
标题:AKR2A participates in the regulation of cotton fibre development by modulating biosynthesis of verylong- chain fatty acids
影响因子:6.840
检测指标:IAA、H2O2流速
检测样品:棉纤维尖端
IAA、H2O2流实验处理方法:15 DPA
IAA、H2O2流实验测试液成份:0.1 mM KCl, 0.1 mM CaCl2, 0.1 mM MgCl2, 0.5 mM NaCl,0.3 mM MES, 0.2 mM Na2SO4 and 0.1% sucrose, pH 6.0
作者:中国农科院棉花所李付广、浙江省农科院沈国新

英文摘要

The biosynthesis of very‐long‐chain fatty acids (VLCFAs) and their transport are required for fibre development. However, whether other regulatory factors are involved in this process is unknown. We report here that overexpression of an Arabidopsis gene ankyrin repeat‐containing protein 2A (AKR2A) in cotton promotes fibre elongation.

RNA‐Seq analysis was employed to elucidate the mechanisms of AKR2A in regulating cotton fibre development. The VLCFA content and the ratio of VLCFAs to short‐chain fatty acids increased in AKR2A transgenic lines. In addition, AKR2A promotes fibre elongation by regulating ethylene and synergizing with the accumulation of auxin and hydrogen peroxide. Analysis of RNA‐Seq data indicates that AKR2A up‐regulates transcript levels of genes involved in VLCFAs’ biosynthesis, ethylene biosynthesis, auxin and hydrogen peroxide signalling, cell wall and cytoskeletal organization. Furthermore, AKR2A interacted with KCS1 in Arabidopsis both in vitro and in vivo. Moreover, the VLCFA content and the ratio of VLCFAs to short‐chain fatty acids increased significantly in seeds of AKR2A‐overexpressing lines and AKR2A/KCS1 co‐overexpressing lines, while AKR2A mutants are the opposite trend.

Our results uncover a novel cotton fibre growth mechanism by which the critical regulator AKR2A promotes fibre development via activating hormone signalling cascade by mediating VLCFA biosynthesis. This study provides a potential candidate gene for improving fibre yield and quality through genetic engineering.

 

中文摘要(谷歌机翻译)

超长链脂肪酸(VLCFA)的生物合成及其运输是纤维发展所必需的。但是,此过程是否涉及其他监管因素尚不清楚。我们在此报告,棉花中拟南芥基因锚蛋白重复序列蛋白2A(AKR2A)的过表达促进了纤维的伸长。

RNA‐Seq分析用于阐明AKR2A调节棉纤维发育的机制。在AKR2A转基因品系中,VLCFA含量和VLCFA与短链脂肪酸的比率增加。另外,AKR2A通过调节乙烯并与植物生长素和过氧化氢的积累协同作用来促进纤维伸长。RNA-Seq数据分析表明,AKR2A上调参与VLCFAs生物合成,乙烯生物合成,生长素和过氧化氢信号,细胞壁和细胞骨架组织的基因的转录水平。此外,在体外和体内,AKR2A与拟南芥中的KCS1相互作用。此外,AKR2A过表达品系和AKR2A / KCS1共过表达品系的种子中VLCFA含量和VLCFA与短链脂肪酸的比率显着增加,而AKR2A突变体则相反。

我们的结果揭示了一种新颖的棉纤维生长机制,关键调节剂AKR2A通过介导VLCFA生物合成,通过激活激素信号传导级联来促进纤维发育。这项研究为通过基因工程提高纤维产量和质量提供了潜在的候选基因。

 

结果表明:WT中IAA的平均外排速率为4262.30 fmol·cm-2·s-1;而AKR2A-2和AKR2A-57的外排速率平均值分别是14298.01 fmol·cm-2·s-1和13685.83 fmol·cm-2·s-1,显然AKR2A-OE的IAA外排速率明显高于WT。
WT中H2O2的平均外排速率为0.02 pmol·cm-2·s-1;而AKR2A-2和AKR2A-57的吸收速率平均值分别是0.49 pmol·cm-2·s-1和0.80 pmol·cm-2·s-1
相比于WT,AKR2A-OE的IAA外排速率和H2O2吸收速率在纤维伸长过程中明显增加。

文章链接:https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.13221